Fig. 4

Results of AIDD pathway expression analysis. a Gene Ontology term “innate immune system” shows clustering of ZIKV infected cells with the CSZ phenotype (K048 & K054) and clustering of normal phenotype (G010) with the mock infected cells of all 3 cell lines. b Customized “interferon alpha pathway” list shows similar clustering pattern as (a). The CZS phenotype cell lines (K048 & K054) show the top 10 differentially expressed genes with gene products induced by interferon alpha pathway, including OAS1 and 2, and intermediary genes in the interferon alpha pathway, including STAT1 (c, d, respectively). On the other hand, phenotypically normal cell line (G010) has only 2 differentially expressed genes, which are not part of the interferon alpha pathway (e). Gene ontology terms “brain development” (f), “CNS development” (g), “neurological development” (h), and “PNS development” (i) exhibit differential expression patterns that can be attributed to genetic differences among cell lines, but not associated with ZIKV infection