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Fig. 3 | BMC Bioinformatics

Fig. 3

From: FocAn: automated 3D analysis of DNA repair foci in image stacks acquired by confocal fluorescence microscopy

Fig. 3

Time-courses of DNA DSB induction and repair in two glioblastoma cell lines, DK-MG and SNB19 (red and blue symbols, respectively). The cells were irradiated with 2 Gy, fixed at the indicated time intervals after irradiation, immunolabeled for γH2AX and examined by 3D confocal microscopy. Each data point represents the mean (±SE) foci number per nucleus of at least 80 cells. The 3D foci numbers were acquired automatically from the image stacks using FocAn. The total computation time for the depicted data was ~ 30 h. The inset shows γH2AX foci counts during the first 3 h after irradiation in detail. The lines are best fits of the modified Mariotti-model (Eq. 4; for detail see text and [33]) to the experimental data

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BMC Bioinformatics

ISSN: 1471-2105

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