Fig. 3

Brick plots enable visualization of immune cell populations. Data used was acquired by mass cytometry from CRC patient cohort 2 (pooled, Additional file 6). a-f Brick plots generated downstream of x-shift clustering in VorteX. (A) all marker locations defined by PCA and coloured by K-means cluster assignation – pink represents markers associated with T cells, orange represents markers associated with resident myeloid cells, light purple represents markers associated with NK cells and dark purple represents markers associated with activated myeloid cells. b-f Brick size indicates relative expression of the labelled marker, colour indicates marker cluster assignation. g SCAFFoLD plot generated using the same dataset, with grid reference overlay added post-analysis. Blue dots indicate predefined landmark nodes, grey (low) to red (high) dots indicate relative CD25 expression. The size of each node indicates relative population size. The purple box outlines the CD25hi myeloid populations depicted in (f)