Fig. 2

Analysis of BiGMeC prediction accuracy for eight selected BGCs. a Barplot showing the number of correct domains when comparing BiGMeC-constructed pathways with pathways as they are detailed in the literature (Additional file 2). The filled part of each bar, as well as the ratio printed above, shows the number of correct domains for each BGC. Extending domains comprise the domains that append an extender unit to the polyketide or peptide backbone, while the non-extending domains cover all other domains. b Predicted maximum production rate when introduced into a S. coelicolor GEM. The x- and y-axis represent the maximal production rate using the metabolic pathway created based on literature or reconstructed with BiGMeC, respectively. c This panel shows a comparison of the predicted reaction-knockout targets (x-axis) when using a metabolic pathway created based on literature or with BiGMeC. Similar predictions are shown as green tiles, while incorrect predictions (predicted in either but not both of the two cases) are shown as red tiles. The names of the model reaction IDs are: TKT1: transketolase; ASPT: aspartate ammonia-lyase; FERO: ferroxidase; GLYCL: glycine cleavage system; MCOALY: malyl-CoA lyase; AGT: alanine-glyoxylate aminotransferase; FUM: fumarase; ASPTA: aspartate transaminase; CITMS: (R)-citramalate synthase; ERTHMMOR: 3-isopropylmalate dehydrogenase; CITCIa2: (R)-2-Methylmalate hydro-lyase; CITCIb: 2-methylmaleate hydratase; GHMT2r: glycine hydroxymethyltransferase; PSERT: phosphoserine transaminase; PGCD: phosphoglycerate dehydrogenase; PSP_L: phosphoserine phosphatase; FDH: formate dehydrogenase