Fig. 2

First-order statistical analysis of the age-associated changes in the cytoplasmic texture of C. elegans oocytes. a Algorithm for calculating the Max–min Value (Mm Value), which is the mean of the difference between the maximum and minimum intensities within each moving window. b–d Comparison of the first-order statistical features of b Mm Value, c SD, and d entropy (3 × 3-pixel window) between Day 1, Day 2, and Day 3 oocytes. Circles indicate individual animals (n = 12 animals in each age group, pooled from two experiments); red bars indicate the mean values. Error bars indicate SEM. Asterisks indicate statistical significance (*P < 0.05; **P < 0.01; Tukey–Kramer test). e–g Comparison of e Mm Value, f SD, and g entropy between Day 1, Day 2, and Day 3 oocytes by using window sizes of 3 × 3 to 29 × 29 (n = 12 animals in each age group, pooled from two experiments). Symbols indicate statistical significance (Tukey–Kramer test) between Day 1 and Day 3 oocytes (*P < 0.05; **P < 0.01) or Day 2 and Day 3 oocytes (^†P < 0.05). Error bars indicate SEM