Fig. 3

Genome-wide spliced-leader (SL) trans-splicing patterns among 13 SLs and five RNA-Seq libraries in Trichuris muris. Top right: generalized PCA of normalised genome-wide SL read counts. Symbol shape represents a priori SL type (circle: SL1; square: SL2) and colour represents cluster membership inferred via K-means clustering (dark grey: cluster1; orange: cluster2). Numbers inside symbols refer to library identifiers as detailed in the dendrogram on the left (hierarchical Ward’s clustering of PCA eigenvectors). Bottom right: linear discriminant analysis between the two clusters, highlighting complete cluster differentiation by the discriminant function (LD1). Tmu-SL1, Tmu-SL4 and Tmu-SL12 are correctly identified as distinct from all other SLs, confirming their functional specialisation as SL2-type SLs