From: PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants
Tool | PnB Designer | SNP-CRISPR | BE-FF | beditor | BE-Designer | Benchling |
---|---|---|---|---|---|---|
pegRNA design for prime editing | ✓ |  ×  |  ×  |  ×  |  ×  |  ×  |
Protospacer design for prime editing | ✓ | ✓ |  ×  |  ×  |  ×  |  ×  |
Integrated PAM variants for ABE | 7 | 0 | 6 | 6 | 6 | 0 |
Assessment of off-targets |  ×  | ✓ | ✓ | ✓ | ✓ | ✓ |
Support multiple sample file import | ✓ | ✓ |  ×  | ✓ | ✓ |  ×  |
Explanatory feedback after wrong input | ✓ | ✓ |  ×  | ✓ |  ×  | ✓ |
Guide design on non-reference genome | ✓ | ✓ | ✓ |  ×  |  ×  |  ×  |
Access | Webserver | Webserver | Webserver | Installation in python environment and GUI or command line | Webserver | Integrated feature on the benchling website |
Input format | Genomic coordinates, text format sequence input by user | Genomic coordinates file only | SNP ID, genomic coordinates, text format sequence input by user | Genomic coordinates file only | Genomic coordinates file, text format sequence input by the user | DNA sequence on the benchling website |
URL | fgcz-shiny.uzh.ch/PnBDesigner/ | flyrnai.org/tools/snp_crispr | danioffenlab.com/be-ff | pypi.org/project/beditor | rgenome.net/be-designer/ | benchling.com |
Reference | – | Chen et al. [38] | Rabinowitz et al. [18] | Dandage et al. [19] | Hwang et al. [20] | – |