Fig. 6

Logo representation of sequences for each individual HHD, extracted from the overall alignment of all HHDs to maintain column correspondence. Clusters corresponding to each HHD were filtered using CD-hit at 95% identity threshold to remove redundant sequences and increase diversity. Number of remaining sequences: 68 RTEL (Viridiplantae), 54 RTEL (Insecta), 83 RTEL HHD1 (Bilateria), 73 RTEL HHD2 (Bilateria), 78 CCM2, 65 Delphilin HHD1, 23 Harmonin, 42 Delphilin HHD2, 33 Whirlin HHD1, 48 PDZD7, 91 Whirlin HHD2. Grayed positions are represented in less than 80% of the sequence hits, non-colored positions are represented in most sequences (>80%), but are not conserved (< 75%) in the final set. Colored positions are conserved in more than 75% of sequences, yellow for hydrophobic, blue for cationic, red for anionic, green for polar and non-charged residues and orange for prolines and glycines. Residues were grouped as follow for conservation calculation: [AVILM], [FY], [TS], [QN], [ED], [RKH], [W], [C], [P], [G]. Recurrent charge-charge and polar interactions are indicated by colored and dashed lines, respectively