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Fig. 1 | BMC Bioinformatics

Fig. 1

From: FINDER: an automated software package to annotate eukaryotic genes from RNA-Seq data and associated protein sequences

Fig. 1

FINDER workflow. FINDER assembles short reads from RNA-Seq expression data, collected from multiple tissues and conditions, to generate full-length transcripts using PsiCLASS. Short read coverage profile is used to polish the structure of the transcripts to enhance the quality of annotation. GeneMarkS-T is used to predict coding regions of the transcripts. Gene models predicted by BRAKER2 and models obtained by mapping proteins are added to the gene models constructed from RNA-Seq data. Additionally, FINDER outputs the tissues where each transcript is expressed allowing users to work with tissue-specific transcripts. FINDER categorizes transcripts into two confidence levels depending on the available supporting evidence and depth of coverage. (Generated using Microsoft PowerPoint v16.47)

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BMC Bioinformatics

ISSN: 1471-2105

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