Fig. 4

a, c, d Examples of customizable graphs for a refueled run: the metrics (yield, speed and median size) are calculated (sum or average) in ten minutes slices and displayed in function of the elapsed time. b Number of reads (color scale) of a given quality (y axis) generated during the sequencing experiment (x axis). Dotted black lines have been added manually and highlight the refueling time (1200, 1650 and 2450 mn). The plots show a drop of the median read size (d) and quality (b) after 500 mn of sequencing at a speed behind 260 b/s (c). The first refuel at 1200 mn restores initial speed (c) and median size (d) but also an almost initial yield per ten minutes (400 Mb vs. 460 Mb, plot a). The last two refuels were made when reaching a translocation rate around 300 b/s as recommended by ONT support