Fig. 2

The Multi-Dimensional Scaling (MDS) plot and Correlogram’ plot. (A)This figure shows the MDS plot generated by RNAdetector in its final report. CRC samples are indicated with blue triangles while the adjacent normal tissue samples are indicated as red circles. MDS allows to perform quality control analysis and it can be interpreted as follows: when the distance among samples of the same biological condition in the MDS space is small, this is an indication of high correlation among them. A larger distance indicates a low correlation and reproducibility among samples. In this case, with the exception of two samples, CRC and normal tissue samples form two distinct clusters (samples are named with their SRR identifiers). (B) This figure shows a ‘correlogram’ plot generated by RNAdetector in its final report. Samples are hierarchically clustered and the correlations between samples are presented as ellipses inside each cell. Each cell represents a pairwise comparison and each correlation coefficient is represented by an ellipse whose ‘diameter’, direction, and color depict the accordance for that pair of samples. Highly correlated samples are depicted as ellipses with narrow diameters, while poorly correlated samples are depicted as ellipses with wide diameters. From the correlogram plot is evident how CRC and normal tissue samples form two distinct groups (samples are named with their SRR identifiers)