Fig. 2

The GLEANER workflow. The workflow guides users through distinct steps of analysis in GLEANER. Users can provide a gene or a genomic region in the human or mouse genome. Once a genomic region was provided, the gene covering the region was regarded as the input gene. In the query step, the transcriptional and epigenome features of the gene in the database are selected as output. In the processing step, the PCCs between gene expression and epigenome features at each developmental phase and the whole germline cycle are calculated using R. The candidate genes with correlated changes can be selected by the users with the satisfying of the thresholds of both PCC and p-value. Then, the polynomial regression model construction of gene expression and each epigenome feature will be performed by the ECharts Statistics module (right). Ultimately, all integrative analysis results will be displayed in three parts, which include integrated analysis plots of transcriptional and epigenetic features, correlated gene expression selection, and visualization of continuous expression and epigenetic features (left)