Fig. 1

Comparison of transcriptome profiles between reference-free (RF) and reference-based (RB) methods. a Proportion of the number of expressed genes identified by RF only, RB only, or both methods. Genes with an FPKM (fragments per kilobase per million reads mapped) value ≥ 1 are annotated as expressed. b Expression level differences of commonly identified genes from both RF and RB methods. The central line and lower and upper edges of the box indicate the median and 25th and 75th percentiles, respectively. The whiskers extend to the furthermost point within 1.2 times the interquartile range (IQR). The p values shown were calculated using Mann–Whitney U test