Fig. 5

Prediction of uORFs in three zebrafish developmental stages. a Left panel: Number of candidate uORFs identified from sequence per stage. The union group describes the union set of all three timepoints. uORFs are colored whether they are common to all stages (green), shared between multiple (blue), or unique to one stage (brown). Right panel: Number of uORFs predicted to be actively translated. Direct comparison of overlapping uORFs between stages can be seen in Additional file 1: Fig. S12. b Distribution of start codons for candidate uORFs (gray) and predicted translated uORFs (purple). c Metrics used as features in classification (x-axis) shown for uORFs predicted to be translated (purple), predicted not to be translated (green), and protein-coding CDSs (yellow). See Additional file 1: Table S1 for a description of features. Y-axis pseudo-log2 score (abs(log2(score)) if score > 0.01, − log2(-score) if score < −0.01, and 0 if absolute value of score is < 0.01)