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Table 2 Summarizing features of the 3 splicing analyses tools

From: Computational comparison of common event-based differential splicing tools: practical considerations for laboratory researchers

  rMATS MISO SUPPA2
Version 4.0.2 0.5.4 2.3
Release Date May 2018 July 2017 February 2018
Other required tools None Samtools Kalisto/Salmon
Reference GTF GFF3 GTF
Input file FASTQ/BAM BAM TPM
Supported experiments Two groups, paired 1 versus 1 comparison Two groups, paired
Job time  ++  – –  + 
RAM  + +  – –  + + 
CPU  + +  – –  + + 
Ease of use  + +  – –
FPGS 8PR detection +/−  +   +/−
  1.  ++ , very good; + , good;  +/− , average; –, poor; ––, very poor