Fig. 1

PhyliCS logical schema. PhyliCS allows to perform downstream analysis on the scCNA profiles computed with scCNA third party callers. Specifically, it accepts tabular data and allows to perform data filtering, feature selection, dimensionality reduction, to prepare the data before executing one of the multiple available clustering algorithms. It also allows to perform clustering result quality evaluation by means of both internal and external evaluation metrics. But, most importantly, it provides the possibility to aggregrate scCNA data from multiple samples, to jointly cluster and visualize them, estimating their spatial ITH through the SHscore