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Fig. 6 | BMC Bioinformatics

Fig. 6

From: Analytics and visualization tools to characterize single-cell stochasticity using bacterial single-cell movie cytometry data

Fig. 6

Cell life visualization. The user can monitor how a selected cell life evolves in the single-cell movie and detect tracking or segmentation errors. The chosen cell here is the red cell in the magnified area of Fig. 5. In a, corresponding to the correct FLT of Fig. 5a, the red mother cell divides between t = 15 min and t = 20 min as noted in a green box, i.e., the red trajectory terminates at t = 15 min and two daughter cells (green and blue) emerge in its space at t = 20 min. The red cell instances up to t = 15 min correspond to the 4 red nodes in the red subtree of Fig. 5a. After the division event, the green and blue cell instances correspond to the 9 green and 9 blue nodes of the respective subtrees in Fig. 5a. In b, corresponding to the FLT of Fig. 5b, we observe the missing red cell division (red box); the red cell trajectory continues, along with only one (green) daughter cell, after t = 15 min while the blue cell is missing. The red cell instances correspond to the 13 red nodes of the incorrect subtree in the magnified area of Fig. 5b. The green cell instances correspond to the 9 green nodes of the unmatched isolated branch shown in the right bottom part of Fig. 5b

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