Fig. 1

Overview of Circall for the discovery of circRNA from RNA-seq: (i) CircRNA candidate detection to discover a list of circRNA candidates. This includes extraction of unmapped reads, collection of BSJ reads, generation of pseudo-sequences of circRNAs and potential tandem RNAs, and filtering to get the list of candidates; (ii) Statistical assessment to rank the candidates. The contour map is an example from the Hela dataset for the statistics from the permutation in the 2d local false discovery rate (2dfdr) method. The red dots and blue triangles indicate the depleted and non-depleted circRNAs, respectively. The circRNAs with fdr2d are marked by grey squares. Details are described in the main text