Fig. 1

Exploration of impact of proportionality constant on predicted flux in WT and two mutant strains overexpressing NOX and ATPase, respectively, in E. coli (Holm dataset): A growth, B WT acetate secretion, C NOX acetate secretion, D ATPase acetate secretion. Data from growth simulations in (A) are used to identify optimal values for the proportionality constant as those for which model predictions match experimental values (119.8, 141.90 and 175.4 for ATPase, NOX and WT strains respectively). Simulations of acetate production (B, C, D), are done by fixing the fitted values followed by sampling of the solution space. The error bars indicate the standard deviations