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Fig. 4 | BMC Bioinformatics

Fig. 4

From: Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network

Fig. 4

Schematic of GUV stack analysis. a Individual section images at different heights, or z positions are combined to construct a single whole GUV stack. The (x, y) positions of the centers are used to determine whether the z-section images are from the same vesicle. b Filtering strategies can remove vesicles that do not strictly meet the quality to proceed with the final analysis. Individual circular image filtering makes decisions based on the quality of each circular section image. For example, a vesicle with too much multi-lamellar intensity can be removed. GUV stack filtering makes decisions based on the quality of a GUV stack as a unit, such as an insufficient number of images, to reconstruct a whole GUV

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