Fig. 7

Results of the example data analysis by the automated intensity trace calculation. The fluorescence signals from the membrane-bound GFP proteins were quantified before and after the introduction of an inhibitor to detach the proteins from the membrane. The decrease in GFP intensity on the membrane is clear. Each average was calculated from 90 < n GUV samples analyzed from seven image stacks. Error bars indicate standard deviations between image stacks. The difference is statistically significance with P < 0.0001