Fig. 5

Left: degradation rates estimated from a single sample plotted against degradation rates published in [27] (obtained using slam-seq). The red line is obtained through weighted linear regression. The weights are set as \(1-r_{l}\) as indicated by the transparency of the dots. The (weighted) correlation of 55% indicates that the estimated rates are meaningful. Only genes with a mean exon TPM above 100 are taken into account. Right: Correlation between degradation rates obtained by [27] and the ones obtained our single-sample method as a function of expression level. Each line represents a biological replicate. The red dot corresponds to the data shown on the left. As expected, the correlation is higher for highly expressed genes, as the intro to exon ratios can be more reliably estimated. In this experiment, replicate 1 correlates better than the two others, indicating that it is probably of better quality