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Table 1 Basecalling accuracy computed using percent identity and number of unaligned reads across datasets for Guppy 4.4.0, Taiyaki 5.0, and RODAN 1.0

From: RODAN: a fully convolutional architecture for basecalling nanopore RNA sequencing data

Dataset

Basecaller

Median Accuracy

Unaligned

Human [19]

Guppy

90.60

N/A

Taiyaki

91.16

900

RODAN

93.23

1307

Mouse [29]

Guppy

87.65

N/A

Taiyaki

86.25

3079

RODAN

87.99

2819

Arabidopsis [28]

Guppy

91.59

N/A

Taiyaki

91.10

957

RODAN

92.89

1001

Poplar [32]

Guppy

90.16

N/A

Taiyaki

89.72

1598

RODAN

91.11

1652

Yeast [30]

Guppy

91.35

N/A

Taiyaki

90.01

2721

RODAN

91.41

3035

  1. For each dataset, the best result is shown in bold
  2. Each dataset contains 100, 000 reads. Only reads alignable by Guppy were used to build each dataset, hence the N/A for unaligned reads. Additional basecalling statistics are provided in Additional file 1: Table S2