Fig. 3

Mapping and quantification of mature tRNAs. The reads that mapped to the tRNA-masked genomic region are classified as “non-tRNA” (purple bars). The reads that mapped to the premature tRNA region of the additional chromosome are classified as “premature tRNA” (blue bars). The reads that mapped to the pure tRNA gene sequence are classified as “mature tRNA” (green bars). Only cytosolic and highly confident tRNAs are used for the final quantification of mature tRNAs. The numbers below each individual tRNA, isodecoder, and isoacceptor represent the mapped read counts