Fig. 5

Detection of hypoxia in TCGA transcriptomes and tumor sections. A Proportion of TCGA tumor samples classified as hypoxic by an ensemble of the 20 decision trees with higher F₁-score, by primary site. B–D Spatial Gene Expression datasets were downloaded from from 10X Genomics and used as input for the tree classifiers to detect hypoxic regions. On the left column, the expression of ENG, and PECAM1 are highlighted as endothelial markers, while on the right the expression of PDK4, LDHA, and CA9 mark regions of active anaerobic glycolysis. The central column represents the probability of each spot to be classified as hypoxic. B Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE). C Human Glioblastoma. D Human Colorectal Cancer