Fig. 1
From: Statistical evidence for the presence of trajectory in single-cell data
Overview of data transformation steps and trajectory inferential statistics. Characterization of the presence of trajectories is done in a vector space. For example, each point can represent a single cell and the axes expression levels of genes in single cells. The data are first clustered, and an MST is then built on cluster centers. The number next to each node is the degree of the node. The edges highlighted in orange form a longest path in the MST. Three statistics are obtained: (1) \(D_1\), the number of leaf nodes is 4, (2) \(D_2\), the number of degree-2 nodes is 4, and (3) \(L_{\max }\), the length of a longest path in the MST is 7