Fig. 6

Experimental results: a RNA-seq and 3′-end-seq read coverage plots of the 3′-UTR in Srsf3 and Rpl22 gene in the two samples with isoform annotation. b The level of total, short 3′-UTR, and long 3′-UTR transcripts from Srsf3 and Rpl22 was measured by qPCR. Because it is not possible to design specific primers for the qPCR analysis of short 3′-UTR transcript, the amount of short 3′-UTR transcripts were calculated by subtracting the quantity of long 3′-UTR transcripts from total