Fig. 4
From: circGPA: circRNA functional annotation based on probability-generating functions
An example of a graph produced by the EnrichmentMap plugin of the Cytoscape program using the 40 most likely annotations of hsa_circ_0000228. The labels were moved manually so that they do not overlap. Several red circles that correspond to annotation terms with lower p-values can be noticed. Besides that, there are several clusters of terms that share genes. The biggest is located in the top left corner showing a set of terms connected with the reproductive and urinary systems. This indicates that the circular RNA might be connected with those systems. According to circBase [40], the sequence of hsa_circ_0000228 is located on the ZEB1 gene. According to the NCBI summary of publication [41], the ZEB1 gene shows the highest expression in the endometrium (out of 27 tissues presented) and is also highly expressed in the urinary bladder, placenta and prostatic tissues. Also, a recent publication has shown a connection between hsa_circ_0000228 and cervical cancer [42]. circGPA predicts a link of the circRNA to cancer as well, given the fact that HP_SOFT_TISSUE_SARCOMA is the term with the lowest p-value