Fig. 3
From: PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAs
Generated primer pairs from PrimedSherlock alongside background genomic sequences and target sequences were utilized as input for PrimedSherlock. Consensus amplicons were generated to determine valid crRNA targets specific to each virus. Discovered crRNA targets were analyzed for strain coverage and potential off target recognition. Scoring was performed to determine the best two primer & crRNA pairs