Fig.7
From: Pro-MAP: a robust pipeline for the pre-processing of single channel protein microarray data
Pro-MAP pipeline. a Raw array files (of any type) are uploaded to Pro-MAP, b the shiny automatically detects foreground and background intensities and several annotations all of which can be manually edited by the user, c Metadata can be uploaded to the shiny for differential expression and data consolidation, control and/or empty spots to be removed at the consolidation stage can be picked from a drop down menu or an annotated spots file can be uploaded and spots to be removed can be chosen based on annotations, d data is background corrected and normalized, array weights are calculated, and data is consolidated and downloadable; plots are generated, e a limma differential expression analysis, using array weights, is run to identify potential biomarkers and these results are available as an expression set or txt file; a hierarchical cluster of differentially expressed proteins is generated and downloadable for the user. The figure was created using Affinity designer