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Fig. 6 | BMC Bioinformatics

Fig. 6

From: Reverse engineering environmental metatranscriptomes clarifies best practices for eukaryotic assembly

Fig. 6

Functional annotation findings from eukrhythmic reassembly of the simulated raw reads from the designer metatranscriptomes. A summary of KO recovery, showing the total number of KOs that are recovered by the eukrhythmic reassembly that were present in the original transcriptomes “Match”, those that were in the eukrhythmic reassemblies that were not present in the original designer set “false positives”, and those that were present in the designer assemblies but not recovered by eukrhythmic “not recovered”. B the number of occurrences of each KO is compared between the designer metatranscriptomes (horizontal axis) and the eukrhythmic reassemblies (vertical axis). The dotted diagonal line indicates the one-to-one line. C this incidences of each KO in the designer assemblies and the eukrhythmic reassemblies are broken up by the individual assemblies that each KO was recovered from (“incidence count” is the number of KOs meeting each category). The majority of all recovered KOs are shown to be recovered by all four assemblers as well as present in the designer metatranscriptomes. Portions of the bar colored in gray indicate that these KOs were recovered by all of the assemblers listed, but were not found in the designer assembly. D Environmental data for KOs from Narragansett Bay as a comparison to Panel C

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