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Table 3 Evaluation results of the modeled vaccine before and after refinement. Comparisons were performed by various servers ERRAT, Verify3D, PROCHECK, and ProSA

From: Immunoinformatics-aided design of a new multi-epitope vaccine adjuvanted with domain 4 of pneumolysin against Streptococcus pneumoniae strains

Server

Before refinement

After refinement

ERRAT

96.30

96.00

Verify3D

80.68

83.39

PROCHECK

Most favoured regions, 91.4%

Most favoured regions, 94.2%

Additional & generously allowed regions, 8.3%

Additional & generously allowed regions, 5.1%

Disallowed regions, 0.2%

Disallowed regions, 0.6%

ProSA

− 9.83

− 10.02