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Table 5 Physicochemical properties of the final vaccine construct

From: Designing multi-epitope vaccine against important colorectal cancer (CRC) associated pathogens based on immunoinformatics approach

Characteristics

Assessment

Number of amino acids

924

Molecular weight

99 KDa

Theoretical pI

8.13

Total number of positively charged residues (Arg + Lys)

105

Total number of atoms

13,973

Chemical formula

C4511H6895N1187O1370S10

Estimated half-life (mammalian reticulocytes, in vitro), (yeast, in vivo), and (Escherichia coli, in vivo)

1.9, 20 and 10 h

Aliphatic index

69.36

Instability index

23.00

Grand average of hydropathicity (GRAVY)

 − 0.564

Solubility

0.460

Antigenicity

0.8952%

Allergenicity

Non-Allergen