Fig. 1

A t-SNE plot showing manually annotated human, mouse, and rat renal macrophages. B–D Transcriptional divergence in resident and infiltrated macrophages between species. Fold-change values of all expressed genes (with one-to-one orthologs) between resident and infiltrated macrophages in pairs of species (human-mouse, mouse–rat and human–rat pairs). Spearman correlations between all expressed one-to-one orthologues are shown in black, and Spearman correlations between the subset of DEGs (FDR-adjusted P-value < 0.05 in both species) appear in red. Genes that are differentially expressed (FDR-adjusted P-value < 0.05) in both species and corresponding spearman correlation coefficients are in red, differentially expressed in only one species are in blue, and genes that are not differentially expressed are in black. Spearman correlation coefficients of all genes are in black. E Dendrogram based on the fold-change between resident and infiltrated macrophages of all expressed genes across one-to-one orthologs in human, mouse and rat