Fig. 4From: Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies’ MinION deviceSubclonal variant template detection sensitivity. For three different mutation classes (SNV, insertion, deletion), the minimum percent abundance of variant reads (x-axis) that allowed detection of the mutation in the in silico generated mixed datasets is plotted. SNVs and insertions are detectable when around 30% of reads are from the variant template, while deletions were not detectable until 95% of the reads were from the variant templateBack to article page