Table 7 Steps for mapping of circular RNA query onto lncRNA reference dataset
Mapping steps | Executable used | Parameter | Value of parameters |
|---|---|---|---|
Creation of BLAST database | makeblastdb | -in | lncRNA database sequences |
-input_type | 'fasta' | ||
-dbtype | 'nucl' | ||
-out | BLAST_generated_lncRNA_database_name | ||
Searching homology | blastn | -query | circular RNAs database sequences |
-task | 'blastn' | ||
-db | BLAST_generated_lncRNA_database_name | ||
-evalue | 1e-05 | ||
-outfmt | 6 | ||
-perc_identity | 100 | ||
-num_threads | 8 | ||
-out | Output_file name | ||
-max_target_seqs | 1 | ||
-max_hsps | 1 |