Fig. 3

Adapted from O’Brien and Bilder 2012 [24]. b Drosophila embryo imaged with SiMView microscopy. Nuclei are labelled with H2B-eGFP (red) and cell membranes are labelled with lyn-tdTomato under the control of the β-actin promoter (green) (N = 93, TPR = 0.988, PPV = 1.0, JI = 0.988). Adapted from Stegmaier et al. 2016 [25], c Average intensity projection of a uniform blastoderm of Tribolium castaneum embryo with H2B-RFP-labelled nuclei and GAP43-YFP-labelled membranes (N = 36, TPR = 0.952, PPV = 0.976, JI = 0.93). Adapted from Benton et al. [26]. d Confocal micrograph showing the expression of different fluorescent proteins in the stem of Arabidopsis thaliana (N = 105, TPR = 0.927, PPV = 0.86, JI = 0.806). Adapted from Federici and Haseloff [27]. First column: Original sample. Second column: filtered Delaunay triangulation of nuclei. Third column: performance metrics calculated by comparing the final result to the manually created ground truth. N, Number of cells; TPR, True Positive Rate; PPV, Positive predictive value. JI, Jaccard Index. Scale is not shown for images as the method is scale invariant