Fig. 2
From: RegiSTORM: channel registration for multi-color stochastic optical reconstruction microscopy
Evaluation of the registration algorithm with multi-color STORM images of fiducial markers only. A Representative STORM images of fiducial markers before and after registration, imaged with 488 and 642 nm excitation. B Quantification of the Target Registration Error (TRE) for A. 1–5 represent different 256 × 256 pixel areas (images) of the same sample. All the fiducial markers detected in the five different areas are combined in total. In the graph the different areas are separated by dashed lines and the «before» box is always on the left and «after» on the right. n1 = 26, n2 = 30, n3 = 40, n4 = 33, n5 = 29, ntotal = 158. C Representative STORM images of fiducial markers before and after registration, imaged with 561 and 642 nm excitation. D Quantification of the TRE for C. n1 = 46, n2 = 37, n3 = 49, n4 = 22, n5 = 60, ntotal = 214. E Representative STORM images of fiducial markers before and after registration, imaged with 488, 561 and 642 nm excitation. F Quantification of the TRE for E. n1 = 92, n2 = 48, n3 = 67, n4 = 57, n5 = 88, ntotal = 352. Two-sided Wilcoxon signed rank test *p < 0.05. Scale bars in A, C and E = 1 μm. In the box plots the box shows the data from lower to upper quartile, with median marked with a line, whereas the whiskers indicate the minimum and maximum