Fig. 3
From: RegiSTORM: channel registration for multi-color stochastic optical reconstruction microscopy
Evaluation of the registration algorithm with STORM images of cell samples with multi-labeled tubulin and fiducials. A Representative STORM images of dual-labeled tubulin before and after registration, imaged with 488 and 642 nm excitation. B Quantification of the Target Registration Error (TRE) of fiducial markers present in the samples in A. n1 = 11, n2 = 12, n3 = 13, n4 = 5, n5 = 10, ntotal = 51. C Representative STORM images of dual-labeled tubulin before and after registration, imaged with 561 and 642 nm excitation. D Quantification of the TRE of fiducial markers present in the samples in C. n1 = 5, n2 = 11, n3 = 15, n4 = 15, n5 = 19, ntotal = 65. E Representative STORM images of triple-labeled tubulin before and after registration, imaged with 488, 561 and 642 nm excitation. F Quantification of the TRE of fiducial markers present in the samples in E. n1 = 27, n2 = 10, n3 = 35, n4 = 6, n5 = 6, ntotal = 84. Two-sided Wilcoxon signed rank test *p < 0.05. 1–5 represent different 256 × 256 pixel areas (images) of the same sample. All the fiducial markers detected in the five different areas are combined in total. In the graphs the different areas are separated by dashed lines and the «before» box is always on the left and «after» on the right. AF = Alexa Fluor. Scale bars in A, C and E = 2 μm. In the box plots the box shows the data from lower to upper quartile, with median marked with a line, whereas the whiskers indicate the minimum and maximum