Fig. 3

Features for CRISPR-GRANT in indel analysis. A Alignment and quantification plot of reads against the reference sequence. Reference was placed as the first sequence and all the other detected reads were aligned with labeled percentile quantification. B Summary bar plot showing the number of different reads processed. Total reads included reads not-mapped and mapped to reference. Reads mapped to reference consisted of modified and un-modified reads. C Frequency distribution of indels along the reference sequence. D Heterozygous alleles (left) could be assigned to each allele using CRISPR-GRANT for quantifying multiple alleles of a given genomic locus. Sample data: sequencing results from SaCas9-KKH editing of the Rho gene in P23H heterozygous mice [19]