Fig. 3

MitoHiFi assemblies often include sequence absent from previous mitochondrial genome assemblies. A For 60 species (Additional file 2: Table S2), the new MitoHiFi assemblies were compared to sequences available for the same species in INSDC databases. For each pair the percent nucleotide identity of the aligned sequences and the proportional size of the MitoHiFi assembly were measured. B Comparison of MitoHiFi and previously published mitochondrial genomes of Tridacna gigas and Tridacna crocea was performed using dotter, and the results were visualised in dotplots. The dotplots show sequence conservation along the diagonal line, while deviations from the diagonal indicate variations such as repeat copies and missing sequences. The MitoHiFi assemblies include multiple copies of repeat sequences, supported by individual long reads, that are likely collapsed in the previously published sequences