Fig. 8

Comprehensive analysis of marker gene CD3D of CXCR6 CD8⁺ T cell. A UMAP plot and Cell Fraction plot show the cell type clustering and cell ratio of hepatic immune cells based on the single-cell sequencing analysis of GSE125188. B A network of the correlation between the cell type and drug response is also shown on the right (datasets are marked in red, cell types are marked in yellow, and drugs are marked in blue). C The heatmap shows association of KLF2 expression and marker genes of CXCR6 CD8⁺ T cell. D, E GSEA enrichment plot of CXCR6 CD8⁺-T-cell marker genes from GSE125188 scRNA dataset and differential genes expressed in CXCR6 CD8⁺-T-cell induced by the drug Isoflupredone. F Matrix plot of signature genes in GSE125188 dataset referring to CXCR6 CD8⁺ T cell and Isoflupredone. G, H Scatter plots show the correlation of CD3D with PDCD1 and CD274 (PDL1). I GSEA-Hallmark analysis on the CD3D-related genes in HCC. J The expression distribution of CD3D in the different grades of LIHC. K, L Comparison of response to ICB treatments in KLF2 high- and low-expression groups in IMvigor210 cohort 2018 and Kim cohort 2019. M, N Prognostic KM curves of high- and low-expression groups of CD3D in IMvigor210 2018 cohort and Kim cohort 2019. O, P ROC curves show the specificity and sensitivity of CD3D response in IMvigor210 cohort 2018 and Kim cohort 2019. *P < 0.05, **P < 0.01, ***P < 0.001