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Fig. 1 | BMC Bioinformatics

Fig. 1

From: MTG-Link: leveraging barcode information from linked-reads to assemble specific loci

Fig. 1

Overview of the MTG-Link pipeline. a Linked-reads whose barcode is observed in flanking regions surrounding the target sequence are extracted, and constitute the read subsample used in the local assembly step. b The local assembly is performed on an extended target, from the k-mer START (left) to the k-mer STOP (right), using the subsample of linked-reads obtained in a. c A quality score is assigned to the assembled sequence according to its alignment against the target flanking sequences. Only the assembled sequences with good quality scores are returned. Otherwise, a new assembly iteration is performed with lower de Bruijn graph parameters

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