Fig. 3

Biomarker Discovery among CD4⁺ T cells, CD8⁺ T cells, and NK cells. A The Biomarker tool shows genes with high expression in one cell type and very low expression in the other cell types, suggesting that they may be potential biomarkers. B Heatmap showing relative expression values of the potential biomarkers’ gene expression for CD4⁺ T cell, CD8⁺ T cell, and NK cell groups. C 2-dimensional t-SNE plot of CD4⁺ T cells, CD8⁺ T cells, and NK cells from healthy controls using the identified potential biomarkers. D 2-dimensional t-SNE plot of CD4⁺ T cells, CD8⁺ T cells, and NK cells from both healthy and diseased groups using the identified potential biomarkers. E 3-dimensional t-SNE plot using the identified potential biomarkers emphasizes separation between clusters