Fig. 9

Learned complexes from the RL pipeline. A The large (39S) subunit of the mitochondrial ribosome is present in the RL-determined complexes, but broken up into multiple complexes, one of which is shown here. Note the presence of the guanine nucleotide exchange factor RCC1L, which is known to be essential for mitochondrial ribosome biogenesis [30]. B RL recapitulates the KICSTOR complex (C12orf66, KPTN, ITFG2, and SZT2), a multiprotein complex known to regulate mTORC1 and cells’ responses to available nutrient levels [31], but finds one additional putative subunit, the uncharacterized transmembrane protein TMCO4. C The exosome RNA processing complex is well-reconstructed by RL, with additional interactions observed to the tRNA synthetase FARSB and to FAM98B, a component of tRNA splicing ligase, consistent with possible associations among these systems [35]. D The WAVE1/WAVE2 protein complexes, known to regulate actin filament and lamellipodia formation [32, 33], are reconstructed by RL, along with evidence for interaction with the uncharacterized protein KIAA1522. Notably, KIAA1522 was recently suggested by Cho and colleagues to bind WAVE and participate in a community of associated actin-organizing proteins [34]