Fig. 5

Excerpt from a genome assembly workflow. Clockwise from top: All files in the current directory are selected. Guesspairs.py distinguishes forward read files from reverse read files based on the unique strings R1 and R2. Only fastq files with the “fq” file extension will appear in output. The output from guesspairs.py is a new blreads object with forward and reverse reads paired in two columns. Where only 1 read file is present, it is treated as a single read file. After setting parameters in the Spades menu, the Run button launches a Python script which constructs the command line to run the Spades pipeline using the selected files