Fig. 1

DRGNs grown on topographical micropatterns have robust differences in neurite morphology. A, B Representative images of rDRGNs grown on unpatterned (A) and topographically micropatterned substrates (B). C Number of Branches per DRGN is decreased in neurons grown on patterned substrates. D Branching Density (Neurite branches per 100 µm length) is decreased in neurons grown on patterned substrates (E) Alignment Index (Total neurite length divided by length in horizontal direction) shows rDRGNs align well to micropatterned substrates. F Alignment Variation (Variance in Alignment Index measurement of each branch from a single neuron) shows less variation in DRGNs grown on micropatterned substrates. n = 120 and 121 neurons for each comparison. Mann–Whitney test shows smaller values for the Patterned condition for each comparison (p < 0.01). Scale bar = 50 µm