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Table 1 Information about the mitochondrial genome assemblers utilized in this study

From: A systematic comparison of human mitochondrial genome assembly tools

Tool

Source repository

Links for docker images available online

Reference genome dependency

Main approach

Programming language

License

Miscellaneous features

Multithreading support

Input data

ARC

https://github.com/ibest/ARC.git

https://hub.docker.com/repository/docker/nirmal2310/arc_docker/

Yes

Mapping to the reference genome followed by de novo assembly.

Python 2

Apache License 2.0

NA

Yes

Paired-End Illumina Fastq Files (Unzipped)

GetOrganelle

https://github.com/Kinggerm/GetOrganelle.git

https://hub.docker.com/repository/docker/nirmal2310/getorganelle_docker/

Yes

Mapping to the reference genome followed by de novo assembly.

Python 3

GNU GPL v3.0

NA

Yes

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

IOGA

https://github.com/holmrenser/IOGA.git

https://hub.docker.com/repository/docker/nirmal2310/ioga_docker/

Yes

Mapping to the reference genome followed by de novo assembly.

Python 2

GNU Affero GPL v3.0

NA

Yes

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

MEANGS

https://github.com/YanCCscu/MEANGS.git

https://hub.docker.com/repository/docker/nirmal2310/meangs_docker/

No

De novo Assembly

Python 3

GNU GPL v3.0

NA

Yes

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

MITObim

https://github.com/chrishah/MITObim.git

https://hub.docker.com/repository/docker/nirmal2310/mitobim_docker/

Yes

Mapping to the reference genome followed by de novo assembly.

Perl

MIT LICENSE

NA

No

Paired-End/Single-End Illumina Fastq Files (Paired-End should be Interleaved and Unzipped)

MitoFlex

https://github.com/Prunoideae/MitoFlex.git

https://hub.docker.com/repository/docker/nirmal2310/mitoflex_docker/

No

De novo assembly

Python 3

GNU GPL v3.0

Genome annotation

No

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

MitoZ

https://github.com/linzhi2013/MitoZ.git

https://hub.docker.com/repository/docker/nirmal2310/mitoz_docker/

No

De novo assembly

Python 3

GNU GPL v3.0

Genome annotation

Yes

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

MToolBox

https://github.com/mitoNGS/MToolBox.git

NA

Yes

Mapping to the reference genome followed by de novo assembly.

Python 2

GNU GPL v3.0

Haplogroup Prediction, Variant Calling

Yes

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

NOVOPlasty

https://github.com/ndierckx/NOVOPlasty.git

https://hub.docker.com/repository/docker/nirmal2310/novoplasty_docker/

Yes

Seed and extend approach

Perl

NA

NA

No

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

(Can be a single gene sequence)

ORG.Asm

https://git.metabarcoding.org/org-asm/org-asm.git

https://hub.docker.com/repository/docker/nirmal2310/org-asm_docker/

Yes

Seed and extent approach

Python 3

CeCILL LICENSE

NA

No

Paired-End/Single-End Illumina Fastq Files (Can be Gzipped)

(Can be a single gene sequence)

  1. Detailed information about the mitochondrial assemblers used in this study has been given in Table 1. The Docker container for MToolBox is unavailable via Docker Hub but can be built locally using the Docker file provided in the GitHub repository: https://github.com/Nirmal2310/Mitochondrial_Benchmarking_study. The information about GitHub commits for the tools has been provided in Additional file 2: Table S6