Fig. 2

Poisson model scoring filters out regions less likely to reflect true RBFOX2 binding events. a Windows with lower adjusted p-values are more likely to overlap with the canonical RBFOX2 motif, GCAUG, and with eCLIP peaks. b Clusters resulting from merging filtered windows reflect a similar trend, with clusters formed from more stringently filtered windows exhibiting a larger overlap with both motif and eCLIP peak presence. c Clusters formed from more stringently filtered windows are more highly enriched for motif and eCLIP presence compared to a null distribution of clusters of comparable sizes randomly shuffled within respective introns and exons. d RBFOX2 clusters with higher scores exhibit higher overlap with the canonical GCAUG motif or RBFOX2 eCLIP peaks. e Among clusters that overlap eCLIP peaks, higher scoring clusters tend to overlap stronger eCLIP peaks. f Representation of the number of clusters by score. g Cluster quality can be boosted immensely by retaining clusters found across multiple biological replicates