Table 1 Input table format required for the WGS fusion validation pipeline
From: Fast and sensitive validation of fusion transcripts in whole-genome sequencing data
Input column | Description | Example |
|---|---|---|
fusion_id | Unique identifier for each fusion transcript | 1 |
sample_id | Unique identifier/barcode for each sample | sample_01 |
path | Full path to the raw WGS files | ~/raw_data/sample1/sample1.bam |
fiveprime_chr | 5′ chromosome | 8 |
fiveprime_strand | 5′ strand | - |
fiveprime_junction | 5′ fusion junction coordinate | 55,013,468 |
fiveprime_search_start | 5′ fusion search region start coordinate | 54,956,927 |
fiveprime_search_end | 5′ fusion search region end coordinate | 55,013,968 |
threeprime_chr | 3′ chromosome | 8 |
threeprime_strand | 3′ strand | + |
threeprime_junction | 3′ fusion junction coordinate | 61,531,139 |
threeprime_search_start | 3′ fusion search region start coordinate | 61,427,495 |
threeprime_search_end | 3′ fusion search region end coordinate | 61,531,639 |